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Loss of m1acp3Ψ Ribosomal RNA Modification Is a Major Feature of Cancer.

Cell reports (2020-05-07)
Artem Babaian, Katharina Rothe, Dylan Girodat, Igor Minia, Sara Djondovic, Miha Milek, Sandra E Spencer Miko, Hans-Joachim Wieden, Markus Landthaler, Gregg B Morin, Dixie L Mager

The ribosome is an RNA-protein complex that is essential for translation in all domains of life. The structural and catalytic core of the ribosome is its ribosomal RNA (rRNA). While mutations in ribosomal protein (RP) genes are known drivers of oncogenesis, oncogenic rRNA variants have remained elusive. We identify a cancer-specific single-nucleotide variation in 18S rRNA at nucleotide 1248.U in up to 45.9% of patients with colorectal carcinoma (CRC) and present across >22 cancer types. This is the site of a unique hyper-modified base, 1-methyl-3-α-amino-α-carboxyl-propyl pseudouridine (m1acp3Ψ), a >1-billion-years-conserved RNA modification at the peptidyl decoding site of the ribosome. A subset of CRC tumors we call hypo-m1acp3Ψ shows sub-stoichiometric m1acp3Ψ modification, unlike normal control tissues. An m1acp3Ψ knockout model and hypo-m1acp3Ψ patient tumors share a translational signature characterized by highly abundant ribosomal proteins. Thus, m1acp3Ψ-deficient rRNA forms an uncharacterized class of "onco-ribosome" which may serve as a chemotherapeutic target for treating cancer patients.

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Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
Trifluoroacetic acid, ReagentPlus®, 99%
Tris(2-carboxyethyl)phosphine hydrochloride, powder
Glycine, suitable for electrophoresis, ≥99%
LookOut® Mycoplasma PCR Detection Kit, Optimized for use with JumpStart Taq DNA Polymerase, D9307.
Guanidine hydrochloride, ≥99% (titration), organic base and chaeotropic agent
HEPES sodium salt, BioPerformance Certified, suitable for cell culture, ≥99.5%
2-Chloroacetamide, ≥98.0% (HPLC)