• Home
  • Search Results
  • Tunable genetic devices through simultaneous control of transcription and translation.

Tunable genetic devices through simultaneous control of transcription and translation.

Nature communications (2020-05-01)
Vittorio Bartoli, Grace A Meaker, Mario di Bernardo, Thomas E Gorochowski
ABSTRACT

Synthetic genetic circuits allow us to modify the behavior of living cells. However, changes in environmental conditions and unforeseen interactions with the host cell can cause deviations from a desired function, resulting in the need for time-consuming reassembly to fix these issues. Here, we use a regulatory motif that controls transcription and translation to create genetic devices whose response functions can be dynamically tuned. This allows us, after construction, to shift the on and off states of a sensor by 4.5- and 28-fold, respectively, and modify genetic NOT and NOR logic gates to allow their transitions between states to be varied over a >6-fold range. In all cases, tuning leads to trade-offs in the fold-change and the ability to distinguish cellular states. This work lays the foundation for adaptive genetic circuits that can be tuned after their physical assembly to maintain functionality across diverse environments and design contexts.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
IPTG, ≥99% (TLC), ≤0.1% Dioxane
Sigma-Aldrich
LB Broth (Miller), Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture
Sigma-Aldrich
Thiamine hydrochloride, reagent grade, ≥99% (HPLC)
Sigma-Aldrich
L-(+)-Arabinose, ≥99%
Sigma-Aldrich
M9, Minimal Salts, 5X, powder, minimal microbial growth medium
Sigma-Aldrich
D-(+)-Glucose, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
Calcium chloride, meets USP testing specifications
Sigma-Aldrich
Kanamycin sulfate from Streptomyces kanamyceticus, meets USP testing specifications, powder
Supelco
Anhydrotetracycline hydrochloride, VETRANAL®, analytical standard