• Home
  • Search Results
  • Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation.

Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation.

Stem cell research (2020-04-20)
Muwan Chen, Muyesier Maimaitili, Susanne Hvolbøl Buchholdt, Uffe Birk Jensen, Fabia Febbraro, Mark Denham
ABSTRACT

We generated an induced pluripotent stem cell (iPSC) line from fibroblasts of a clinically diagnosed 70 year old female Parkinson's disease (PD) patient heterozygous for a pathogenic missense variant (p.G2019S; c. 6055 G > A) in the leucine-rich repeat kinase 2 (LRRK2) gene by using non-integrating Sendai viruses. The DANi-011A iPSC line has a normal karyotype and is free from Sendai viruses. The expression of pluripotent markers in the iPSC line was confirmed by immunofluorescent staining, and we confirmed its ability to differentiate into the three germ layers. The DANi-011A iPSC line can be used for modeling PD and as a drug-screening platform.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
LookOut® Mycoplasma PCR Detection Kit, Optimized for use with JumpStart Taq DNA Polymerase, D9307.