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  • Elasticizing tissues for reversible shape transformation and accelerated molecular labeling.

Elasticizing tissues for reversible shape transformation and accelerated molecular labeling.

Nature methods (2020-05-20)
Taeyun Ku, Webster Guan, Nicholas B Evans, Chang Ho Sohn, Alexandre Albanese, Joon-Goon Kim, Matthew P Frosch, Kwanghun Chung
ABSTRACT

We developed entangled link-augmented stretchable tissue-hydrogel (ELAST), a technology that transforms tissues into elastic hydrogels to enhance macromolecular accessibility and mechanical stability simultaneously. ELASTicized tissues are highly stretchable and compressible, which enables reversible shape transformation and faster delivery of probes into intact tissue specimens via mechanical thinning. This universal platform may facilitate rapid and scalable molecular phenotyping of large-scale biological systems, such as human organs.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1,2-Dimethoxyethane, suitable for HPLC, 99.9%, inhibitor-free
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Acrylamide, for molecular biology, ≥99% (HPLC)
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Eosin Y-solution 0.5% alcoholic, for microscopy
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Dextran sulfate sodium salt from Leuconostoc spp., for molecular biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer