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Chlorophyll a oxygenase (CAO) is involved in chlorophyll b formation from chlorophyll a.

Proceedings of the National Academy of Sciences of the United States of America (1998-10-15)
A Tanaka, H Ito, R Tanaka, N K Tanaka, K Yoshida, K Okada
ABSTRACT

Chlorophyll b is an ubiquitous accessory pigment in land plants, green algae, and prochlorophytes. Its biosynthesis plays a key role in the adaptation to various light environments. We isolated six chlorophyll b-less mutants by insertional mutagenesis by using the nitrate reductase or argininosuccinate lyase genes as tags and examined the rearrangement of mutant genomes. We found that an overlapping region of a nuclear genome was deleted in all mutants and that this encodes a protein whose sequence is similar to those of methyl monooxygenases. This coding sequence also contains putative binding domains for a [2Fe-2S] Rieske center and for a mononuclear iron. The results demonstrate that a chlorophyll a oxygenase is involved in chlorophyll b formation. The reaction mechanism of chlorophyll b formation is discussed.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chlorophyll b from spinach, ≥90% (HPLC), ≤0.5% Chlorophyll a