• Home
  • Search Results
  • The deubiquitinases USP33 and USP20 coordinate beta2 adrenergic receptor recycling and resensitization.

The deubiquitinases USP33 and USP20 coordinate beta2 adrenergic receptor recycling and resensitization.

The EMBO journal (2009-05-09)
Magali Berthouze, Vidya Venkataramanan, Yi Li, Sudha K Shenoy
ABSTRACT

Agonist-induced ubiquitination of the beta(2) adrenergic receptor (beta(2)AR) functions as an important post-translational modification to sort internalized receptors to the lysosomes for degradation. We now show that this ubiquitination is reversed by two deubiquitinating enzymes, ubiquitin-specific proteases (USPs) 20 and 33, thus, inhibiting lysosomal trafficking when concomitantly promoting receptor recycling from the late-endosomal compartments as well as resensitization of recycled receptors at the cell surface. Dissociation of constitutively bound endogenously expressed USPs 20 and 33 from the beta(2)AR immediately after agonist stimulation and reassociation on prolonged agonist treatment allows receptors to first become ubiquitinated and then deubiquitinated, thus, providing a 'trip switch' between degradative and recycling pathways at the late-endosomal compartments. Thus, USPs 20 and 33 serve as novel regulators that dictate both post-endocytic sorting as well as the intensity and extent of beta(2)AR signalling from the cell surface.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Fibronectin bovine plasma, solution, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Triton X-100, BioXtra
Sigma-Aldrich
Fibronectin bovine plasma, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Anti-USP33 antibody produced in rabbit, affinity isolated antibody, buffered aqueous glycerol solution