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MRI assessment of ischemic liver after intraportal islet transplantation.

Transplantation (2009-03-21)
Naoaki Sakata, Pete Hayes, Annie Tan, Nathaniel K Chan, John Mace, Ricardo Peverini, Lawrence Sowers, William J Pearce, Richard Chinnock, Andre Obenaus, Eba Hathout
ABSTRACT

There is a recent focus on embolization of the portal vein by transplanted islets as a major cause of early graft loss. The resultant ischemia causes necrosis or apoptosis of cells within the liver. Thus, noninvasive assessment of the liver receiving the islet transplant is important to evaluate the status islet grafts. This study used noninvasive magnetic resonance imaging (MRI) for assessment of the posttransplant ischemic liver. Syngeneic islets in streprozotocin-induced diabetic mice were used. MRI and morphological liver assessments were performed at 0, 2, and 28 days after transplantation. Histologic assessment of insulin, hypoxia induced factor 1-alpha, and apoptosis were undertaken at similar time points. Ischemic/necrotic regions in the liver were detected by MRI at 2 days but not at 28 days after transplantation and were confirmed histologically. Liver injury was quantified from high intensity areas on T2-weighted images. Insulin release peaked 2 days after transplantation. Onset and reversal of liver ischemia due to intraportal islet transplantation are detectable using T2-weighted MRI. These changes coincide with periods of maximum insulin release likely due to partial islet destruction. We propose that MRI, as a noninvasive monitor of graft-related ischemia, may be useful in assessment of liver and islet engraftment after intraportal islet transplantation in a clinical setting.

MATERIALS
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Product Description

Sigma-Aldrich
Ficoll® solution, Type 400, 20% in H2O
Sigma-Aldrich
Ficoll® 400, BioXtra, for molecular biology, lyophilized powder
Sigma-Aldrich
Ficoll® 400, lyophilized powder, Type 400-DL, BioReagent, suitable for cell culture
Sigma-Aldrich
Ficoll® 400, Type 400-DL, lyophilized powder
Sigma-Aldrich
Ficoll® 400, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
Sigma-Aldrich
Ficoll® 400, BioXtra, Type 400-DL, lyophilized powder
Supelco
Polyethylene (40, 75, 200, 400 mg Cd/kg), certified reference material

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