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Organic Modifications of SBA-15 Improves the Enzymatic Properties of its Supported TLL.

Journal of oleo science (2020-08-08)
Fenghuan Zhao, Xihong Zhao, Nanjing Zhong
ABSTRACT

In this study, lipase from Thermomyces lanuginosus (TLL) was immobilized onto the parent and organic groups modified SBA-15, and the enzymatic properties of the obtained immobilized TLL samples were investigated. 1) Activity of SBA-15-TLL at 2862.78 ± 293.24 U/g was obtained. 2) Most of the organic groups modification favored a great improvement in activity, and higher activity over 12000 U/g was observed for N-phenylaminomethyl and phenyl group modification. 3) Most of the supported TLL showed better thermostability in air while poor in phosphate buffer, with over 80% vers less than 20% of their initial activity retained after 4 h incubation at 70℃. 4) The n-dodecyl, phenyl and N-phenylaminomethyl group functionalization decreased the sensitivity of immobilized TLL in extreme pH values. 5) The n-octyl and 2-(propoxymethyl)oxirane group modification confered the supported TLL good reusability, and over 60% of their initial activity was retained after five successive cycles of reuse.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1,3-Diolein, ≥99% (GC)
Sigma-Aldrich
(3-Mercaptopropyl)triethoxysilane, ≥80% (GC), technical
Sigma-Aldrich
n-Octadecyltriethoxysilane, AldrichCPR