MilliporeSigma
  • Home
  • Search Results
  • VEGFR2 signaling drives meningeal vascular regeneration upon head injury.

VEGFR2 signaling drives meningeal vascular regeneration upon head injury.

Nature communications (2020-08-02)
Bong Ihn Koh, Hyuek Jong Lee, Pil Ae Kwak, Myung Jin Yang, Ju-Hee Kim, Hyung-Seok Kim, Gou Young Koh, Injune Kim
ABSTRACT

Upon severe head injury (HI), blood vessels of the meninges and brain parenchyma are inevitably damaged. While limited vascular regeneration of the injured brain has been studied extensively, our understanding of meningeal vascular regeneration following head injury is quite limited. Here, we identify key pathways governing meningeal vascular regeneration following HI. Rapid and complete vascular regeneration in the meninges is predominantly driven by VEGFR2 signaling. Substantial increase of VEGFR2 is observed in both human patients and mouse models of HI, and endothelial cell-specific deletion of Vegfr2 in the latter inhibits meningeal vascular regeneration. We further identify the facilitating, stabilizing and arresting roles of Tie2, PDGFRβ and Dll4 signaling, respectively, in meningeal vascular regeneration. Prolonged inhibition of this angiogenic process following HI compromises immunological and stromal integrity of the injured meninges. These findings establish a molecular framework for meningeal vascular regeneration after HI, and may guide development of wound healing therapeutics.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-PECAM-1 Antibody, clone 2H8, Azide Free, clone 2H8, Chemicon®, from hamster(Armenian)
Sigma-Aldrich
Anti-PTPRB antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody
Sigma-Aldrich
Anti-Vimentin Antibody, serum, Chemicon®
Sigma-Aldrich
Human VEGF-A ELISA Kit, for serum, plasma, cell culture supernatants and urine
Sigma-Aldrich
Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, Chemicon®, from rabbit