Skip to Content
MilliporeSigma
  • Toxicity Assessment of Titanium (IV) Oxide Nanoparticles Using Daphnia magna (Water Flea).

Toxicity Assessment of Titanium (IV) Oxide Nanoparticles Using Daphnia magna (Water Flea).

Environmental health and toxicology (2011-11-30)
Seung Hyuck Bang, Thai-Hoang Le, Sung Kyu Lee, Pil Kim, Jong Soo Kim, Jiho Min
ABSTRACT

Titanium dioxide (TiO(2)), a common nanoparticle widely used in industrial production, is one of nano-sized materials. The purpose of this study was to determine the acute and chronic toxicity of TiO(2) using different size and various concentrations on Daphnia magna. In the acute toxicity test, four concentrations (0, 0.5, 4, and 8 mM) for TiO(2) with 250 or 500 nm and five concentrations (0, 0.25, 0.5, 0.75, and 1 mM) for TiO(2) with 21 nm were selected to analyze the toxic effect to three groups of ten daphnia neonates over 96 hours. In addition, to better understand their toxicity, chronic toxicity was examined over 21 days using 0, 1, and 10 mM for each type of TiO(2). Our results showed that all organisms died before the reproduction time at a concentration of 10 mM of TiO(2). In addition, the exposure of anatase (21 nm) particles were more toxic to D. magna, comparing with that of anatase (250 nm) and rutile (500 nm) particles. This study indicated that TiO(2) had adverse impacts on the survival, growth and reproduction of D. magna after the 21days exposure. In addition, the number of test organisms that were able to reproduce neonates gradually were reduced as the size of TiO(2) tested was decreased.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Titanium(IV) oxide, nanopowder, 21 nm primary particle size (TEM), ≥99.5% trace metals basis
Sigma-Aldrich
Titanium(IV) oxide, contains 1% Mn as dopant, nanopowder, <100 nm particle size (BET), ≥97%
Sigma-Aldrich
Titanium(IV) oxide, JIS special grade, ≥99.0%
Sigma-Aldrich
Titanium(IV) oxide, ReagentPlus®, ≥99%
Sigma-Aldrich
Titanium(IV) oxide, puriss., meets analytical specification of Ph. Eur., BP, USP, 99-100.5%