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  • Extraction and isolation of the active ingredients of dandelion and its antifungal activity against Candida albicans.

Extraction and isolation of the active ingredients of dandelion and its antifungal activity against Candida albicans.

Molecular medicine reports (2019-11-21)
Yinku Liang, Hongbo Duan, Ping Zhang, Hao Han, Feixiong Gao, Yunxiang Li, Zhongyang Xu
ABSTRACT

In this study, six compounds were isolated and purified from dandelion, and only sample I exhibited notable antifungal effect on Candida albicans (CA). high‑performance liquid chromatography‑diode‑array detector‑electrospray ionization‑tandem mass spectrometry analysis showed that sample I comprised 4‑coumaric acid, ferulic acid, quercetin pentoside, 3,5‑di‑O‑caffeoylquinic acid, 4,5‑di‑O‑caffeoylquinic acid, luteolin, and two unknown compounds, at a relative percent composition of 11.45, 3.96, 10.48, 34.24, 3.91, 11.80, 3.65 and 4.21%, respectively. Further antimicrobial experiments showed that the minimum inhibitory concentration of sample I was 32.0 mg/ml, and sample I mainly acts on bacterial growth in the exponential phase of CA growth. Optical density and infrared analyses conclusively suggested that sample I damages the structure of CA cells, particularly the cell wall and cell membrane, resulting in macromolecule leakage of intracellular nucleic acids and cell metabolism disruption. In conclusion, dandelion sample I was reported to increase CA cell membrane permeability by affecting the glycosidic bond in β‑(1‑3)‑D glucan and destroying the cell wall, ultimately leading CA to death.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
4,5-Di-O-caffeoylquinic acid, ≥85% (LC/MS-ELSD)