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Genomic approaches for interrogating the biochemistry of medicinal plant species.

Methods in enzymology (2012-10-23)
Elsa Góngora-Castillo, Greg Fedewa, Yunsoo Yeo, Joe Chappell, Dean DellaPenna, C Robin Buell
ABSTRACT

Development of next-generation sequencing, coupled with the advancement of computational methods, has allowed researchers to access the transcriptomes of recalcitrant genomes such as those of medicinal plant species. Through the sequencing of even a few cDNA libraries, a broad representation of the transcriptome of any medicinal plant species can be obtained, providing a robust resource for gene discovery and downstream biochemical pathway discovery. When coupled to estimation of expression abundances in specific tissues from a developmental series, biotic stress, abiotic stress, or elicitor challenge, informative coexpression and differential expression estimates on a whole transcriptome level can be obtained to identify candidates for function discovery.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Spectrum Plant Total RNA Kit, sufficient for 10 purifications