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  • Global and gene-specific DNA methylation pattern discriminates cholecystitis from gallbladder cancer patients in Chile.

Global and gene-specific DNA methylation pattern discriminates cholecystitis from gallbladder cancer patients in Chile.

Future oncology (London, England) (2014-07-30)
Luciane Tsukamoto Kagohara, Juliana L Schussel, Tejaswini Subbannayya, Nandini Sahasrabuddhe, Cynthia Lebron, Mariana Brait, Leonel Maldonado, Blanca L Valle, Francesca Pirini, Martha Jahuira, Jaime Lopez, Pablo Letelier, Priscilla Brebi-Mieville, Carmen Ili, Akhilesh Pandey, Aditi Chatterjee, David Sidransky, Rafael Guerrero-Preston
ABSTRACT

The aim of the study was to evaluate the use of global and gene-specific DNA methylation changes as potential biomarkers for gallbladder cancer (GBC) in a cohort from Chile. DNA methylation was analyzed through an ELISA-based technique and quantitative methylation-specific PCR. Global DNA Methylation Index (p = 0.02) and promoter methylation of SSBP2 (p = 0.01) and ESR1 (p = 0.05) were significantly different in GBC when compared with cholecystitis. Receiver curve operator analysis revealed promoter methylation of APC, CDKN2A, ESR1, PGP9.5 and SSBP2, together with the Global DNA Methylation Index, had 71% sensitivity, 95% specificity, a 0.97 area under the curve and a positive predictive value of 90%. Global and gene-specific DNA methylation may be useful biomarkers for GBC clinical assessment.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Imprint® Methylated DNA Quantification Kit, To measure global DNA methylation shifts from as low as 10 ng DNA