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Substrate specificity effects of lipoxygenase products and inhibitors on soybean lipoxygenase-1.

Bioorganic & medicinal chemistry (2009-09-01)
Aaron T Wecksler, Natalie K Garcia, Theodore R Holman
ABSTRACT

Recently, it has been shown that lipoxygenase (LO) products affect the substrate specificity of human 15-LO. In the current paper, we demonstrate that soybean LO-1 (sLO-1) is not affected by its own products, however, inhibitors which bind the allosteric site, oleyl sulfate (OS) and palmitoleyl sulfate (PS), not only lower catalytic activity, but also change the substrate specificity, by increasing the arachidonic acid (AA)/linoleic acid (LA) ratio to 4.8 and 4.0, respectively. The fact that LO inhibitors can lower activity and also change the LO product ratio is a new concept in lipoxygenase inhibition, where the goal is to not only reduce the catalytic activity but also alter substrate selectivity towards a physiologically beneficial product.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Oleic Acid-Water Soluble, powder, BioReagent, suitable for cell culture
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Oleic acid, meets analytical specification of Ph, Eur., 65.0-88.0% (GC)
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Oleic acid, technical grade, 90%
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Oleic acid, natural, FCC
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Oleic acid, Selectophore, ≥99.0%
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Linoleic acid, analytical standard
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Linoleic acid, technical, 58-74% (GC)
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Linoleic acid, ≥99%
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Linoleic acid, liquid, BioReagent, suitable for cell culture