MilliporeSigma
  • Home
  • Search Results
  • Fine-scale visualizing the hierarchical structure of mouse biliary tree with fluorescence microscopy method.

Fine-scale visualizing the hierarchical structure of mouse biliary tree with fluorescence microscopy method.

Bioscience reports (2020-05-05)
Yuwei Chen, Lin Bai, Yongjie Zhou, Xiaoyun Zhang, Jie Zhang, Yujun Shi
ABSTRACT

The liver is a vital organ and the hepatic lobule serves as the most basic structural and functional unit which is mainly assembled with parenchymal cells including hepatocytes and biliary epithelial cells. The continuous tubular arrangement of biliary cells which constitutes the biliary tracts is critical for liver function, however, the biliary tracts are often disrupted in many liver diseases such as cirrhosis and some congenital disorders. Visualization of the biliary tracts in fine-scale and three-dimension will help to understanding the structure basis of these liver diseases. In the present study, we established several biliary tract injury mouse models by diet feeding, surgery or genetic modification. The cytoplasm and nuclei of the parenchymal cells were marked by active uptake of fluorescent dyes Rhodamine B (red) and Hoechst (blue), respectively. After the removal of liver en bloc, the biliary tracts were retrogradely perfused with green fluorescent dye, fluorescein isothiocyanate (FITC). The liver was then observed under confocal microscopy. The fine-scale and three-dimensional (3D) structure of the whole biliary tree, particularly the network of the end-terminal bile canaliculi and neighboring hepatocytes were clearly visualized. The biliary tracts displayed clear distinct characteristics in normal liver and diseased liver models. Taken together, we have developed a simple and repeatable imaging method to visualize the fine-scale and hierarchical architecture of the biliary tracts spreading in the mouse liver.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sulforhodamine B, Dye content 75 %