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Protocol for Microbiome Transplantation in Suckling Mice during Vibrio cholerae Infection to Study Commensal-Pathogen Interactions.

STAR protocols (2020-12-31)
Salma Alavi, Ansel Hsiao
ABSTRACT

The gut microbiome plays an important role in the exclusion of pathogens and, thus, infection outcomes. Microbiome-pathogen interaction studies are complicated by a lack of tractable animal models and differences in animal model versus human microbiomes. We have adapted the suckling mouse model of infection of the human pathogen Vibrio cholerae to clear murine microbes and establish human-associated gut microbes during infection. Our method allows for the easy examination of the contribution of different human microbial communities to enteropathogenesis. For complete details on the use and execution of this protocol, please refer to Alavi et al. (2020).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
D-(+)-Maltose monohydrate, from potato, ≥99%
Sigma-Aldrich
L-Cysteine hydrochloride monohydrate, from non-animal source, suitable for cell culture, meets EP, USP testing specifications