• Home
  • Search Results
  • Microneedle patch for the ultrasensitive quantification of protein biomarkers in interstitial fluid.

Microneedle patch for the ultrasensitive quantification of protein biomarkers in interstitial fluid.

Nature biomedical engineering (2021-01-24)
Zheyu Wang, Jingyi Luan, Anushree Seth, Lin Liu, Minli You, Prashant Gupta, Priya Rathi, Yixuan Wang, Sisi Cao, Qisheng Jiang, Xiao Zhang, Rohit Gupta, Qingjun Zhou, Jeremiah J Morrissey, Erica L Scheller, Jai S Rudra, Srikanth Singamaneni
ABSTRACT

The detection and quantification of protein biomarkers in interstitial fluid is hampered by challenges in its sampling and analysis. Here we report the use of a microneedle patch for fast in vivo sampling and on-needle quantification of target protein biomarkers in interstitial fluid. We used plasmonic fluor-an ultrabright fluorescent label-to improve the limit of detection of various interstitial fluid protein biomarkers by nearly 800-fold compared with conventional fluorophores, and a magnetic backing layer to implement conventional immunoassay procedures on the patch and thus improve measurement consistency. We used the microneedle patch in mice for minimally invasive evaluation of the efficiency of a cocaine vaccine, for longitudinal monitoring of the levels of inflammatory biomarkers, and for efficient sampling of the calvarial periosteum-a challenging site for biomarker detection-and the quantification of its levels of the matricellular protein periostin, which cannot be accurately inferred from blood or other systemic biofluids. Microneedle patches for the minimally invasive collection and analysis of biomarkers in interstitial fluid might facilitate point-of-care diagnostics and longitudinal monitoring.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Bovine Serum Albumin, heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%
Sigma-Aldrich
L-Ascorbic acid, 99%
Sigma-Aldrich
Gold(III) chloride trihydrate, ≥99.9% trace metals basis
Sigma-Aldrich
Ethylene glycol, ReagentPlus®, ≥99%
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, ≥98%
Sigma-Aldrich
Hydrochloric acid solution, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
Iron(III) chloride hexahydrate, ACS reagent, 97%
Sigma-Aldrich
(3-Aminopropyl)trimethoxysilane, 97%
Sigma-Aldrich
(3-Mercaptopropyl)trimethoxysilane, 95%
Sigma-Aldrich
Ethanolamine, ACS reagent, ≥99.0%
Sigma-Aldrich
Sodium acetate trihydrate, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Silver nitrate, 99.9999% trace metals basis
Sigma-Aldrich
Cocaine free base
Sigma-Aldrich
Anti-POSTN antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-POSTN antibody produced in rabbit, affinity isolated antibody