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  • Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry.

Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry.

Developmental cell (2021-05-02)
Jules Lavalou, Qiyan Mao, Stefan Harmansa, Stephen Kerridge, Annemarie C Lellouch, Jean-Marc Philippe, Stephane Audebert, Luc Camoin, Thomas Lecuit
ABSTRACT

Interfaces between cells with distinct genetic identities elicit signals to organize local cell behaviors driving tissue morphogenesis. The Drosophila embryonic axis extension requires planar polarized enrichment of myosin-II powering oriented cell intercalations. Myosin-II levels are quantitatively controlled by GPCR signaling, whereas myosin-II polarity requires patterned expression of several Toll receptors. How Toll receptors polarize myosin-II and how this involves GPCRs remain unknown. Here, we report that differential expression of a single Toll receptor, Toll-8, polarizes myosin-II through binding to the adhesion GPCR Cirl/latrophilin. Asymmetric expression of Cirl is sufficient to enrich myosin-II, and Cirl localization is asymmetric at Toll-8 expression boundaries. Exploring the process dynamically, we reveal that Toll-8 and Cirl exhibit mutually dependent planar polarity in response to quantitative differences in Toll-8 expression between neighboring cells. Collectively, we propose that the cell surface protein complex Toll-8/Cirl self-organizes to generate local asymmetric interfaces essential for planar polarization of contractility.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Penicillin-Streptomycin, Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture
Roche
Anti-HA High Affinity, from rat IgG1
Sigma-Aldrich
20-Hydroxyecdysone, ≥93% (HPLC), powder
Sigma-Aldrich
Grace′s Insect Medium, With L-glutamine, without sodium bicarbonate, powder, suitable for insect cell culture