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Root Aliphatic Suberin Analysis Using Non-extraction or Solvent-extraction Methods.

Bio-protocol (2017-06-20)
Camille Delude, Sollapura J Vishwanath, Owen Rowland, Frédéric Domergue
ABSTRACT

Here we describe both non-extraction and solvent-extraction methods for root aliphatic suberin analysis. The non-extraction method is fast as roots are directly depolymerized using acidic transmethylation. However, suberin aliphatic components are isolated together with all the other acyl chains making up the lipids (e.g., membranes) present in roots. For the solvent-extraction method, roots are first delipidated before transmethylation. This method is longer but allows separation of soluble and polymerized root lipids. This protocol is optimized for tissue culture- or soil-grown Arabidopsis thaliana plants, but can be used with roots of other plants.

MATERIALS
Product Number
Brand
Product Description

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Heptadecanoic acid, ≥98%
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