Skip to Content
MilliporeSigma

Resident cardiac macrophages mediate adaptive myocardial remodeling.

Immunity (2021-07-29)
Nicole R Wong, Jay Mohan, Benjamin J Kopecky, Shuchi Guo, Lixia Du, Jamison Leid, Guoshuai Feng, Inessa Lokshina, Oleksandr Dmytrenko, Hannah Luehmann, Geetika Bajpai, Laura Ewald, Lauren Bell, Nikhil Patel, Andrea Bredemeyer, Carla J Weinheimer, Jessica M Nigro, Attila Kovacs, Sachio Morimoto, Peter O Bayguinov, Max R Fisher, W Tom Stump, Michael Greenberg, James A J Fitzpatrick, Slava Epelman, Daniel Kreisel, Rajan Sah, Yongjian Liu, Hongzhen Hu, Kory J Lavine
ABSTRACT

Cardiac macrophages represent a heterogeneous cell population with distinct origins, dynamics, and functions. Recent studies have revealed that C-C Chemokine Receptor 2 positive (CCR2+) macrophages derived from infiltrating monocytes regulate myocardial inflammation and heart failure pathogenesis. Comparatively little is known about the functions of tissue resident (CCR2-) macrophages. Herein, we identified an essential role for CCR2- macrophages in the chronically failing heart. Depletion of CCR2- macrophages in mice with dilated cardiomyopathy accelerated mortality and impaired ventricular remodeling and coronary angiogenesis, adaptive changes necessary to maintain cardiac output in the setting of reduced cardiac contractility. Mechanistically, CCR2- macrophages interacted with neighboring cardiomyocytes via focal adhesion complexes and were activated in response to mechanical stretch through a transient receptor potential vanilloid 4 (TRPV4)-dependent pathway that controlled growth factor expression. These findings establish a role for tissue-resident macrophages in adaptive cardiac remodeling and implicate mechanical sensing in cardiac macrophage activation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Actin, Cardiac antibody, Mouse monoclonal, clone AC1-20.4.2, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Trpv4 Antibody, clone 1B2.6, clone 1B2.6, from mouse
Sigma-Aldrich
Monoclonal Anti-α-Actinin antibody produced in mouse, clone BM-75.2, ascites fluid