MilliporeSigma
  • An FcRn-targeted mucosal vaccine against SARS-CoV-2 infection and transmission.

An FcRn-targeted mucosal vaccine against SARS-CoV-2 infection and transmission.

Nature communications (2023-11-07)
Weizhong Li, Tao Wang, Arunraj M Rajendrakumar, Gyanada Acharya, Zizhen Miao, Berin P Varghese, Hailiang Yu, Bibek Dhakal, Tanya LeRoith, Athira Karunakaran, Wenbin Tuo, Xiaoping Zhu
ABSTRACT

SARS-CoV-2 is primarily transmitted through droplets and airborne aerosols, and in order to prevent infection and reduce viral spread vaccines should elicit protective immunity in the airways. The neonatal Fc receptor (FcRn) transfers IgG across epithelial barriers and can enhance mucosal delivery of antigens. Here we explore FcRn-mediated respiratory delivery of SARS-CoV-2 spike (S). A monomeric IgG Fc was fused to a stabilized spike; the resulting S-Fc bound to S-specific antibodies and FcRn. Intranasal immunization of mice with S-Fc and CpG significantly induced antibody responses compared to the vaccination with S alone or PBS. Furthermore, we intranasally immunized mice or hamsters with S-Fc. A significant reduction of virus replication in nasal turbinate, lung, and brain was observed following nasal challenges with SARS-CoV-2 and its variants. Intranasal immunization also significantly reduced viral airborne transmission in hamsters. Nasal IgA, neutralizing antibodies, lung-resident memory T cells, and bone-marrow S-specific plasma cells mediated protection. Hence, FcRn delivers an S-Fc antigen effectively into the airway and induces protection against SARS-CoV-2 infection and transmission.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal, clone GG-6, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution