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  • Caldesmon, calponin and alpha-smooth muscle actin expression in subcultured smooth muscle cells from human airways.

Caldesmon, calponin and alpha-smooth muscle actin expression in subcultured smooth muscle cells from human airways.

Histochemistry (1993-12-01)
W Durand-Arczynska, N Marmy, J Durand
ABSTRACT

Calponin and caldesmon are two proteins considered to play a regulatory role in smooth muscle contraction, which have never previously been found to be expressed in subcultured cells. In the present study, immunocytochemistry and immunoblotting were performed to identify these proteins in smooth muscle cells (SMC) from human bronchi. It was found that human airway SMC, kept in a non-proliferative state, continued to express caldesmon and calponin at least until the 8th passage. The expression of alpha-smooth muscle actin studied under the same conditions was also shown to be preserved in subcultured bronchial SMC.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-Actin, α-Smooth Muscle - Peroxidase antibody produced in mouse, clone 1A4, purified from hybridoma cell culture