• Home
  • Search Results
  • Lipid-induced peroxidation in the intestine is involved in glucose homeostasis imbalance in mice.

Lipid-induced peroxidation in the intestine is involved in glucose homeostasis imbalance in mice.

PloS one (2011-06-24)
Matteo Serino, Aurélie Waget, Nicolas Marsollier, Myriam Masseboeuf, Gaëlle Payros, Catherine Kabani, Jessica Denom, Amélie Lacombe, Jean-Claude Thiers, Anne Negre-Salvayre, Serge Luquet, Rémy Burcelin, Céline Cruciani-Guglielmacci, Christophe Magnan
ABSTRACT

Daily variations in lipid concentrations in both gut lumen and blood are detected by specific sensors located in the gastrointestinal tract and in specialized central areas. Deregulation of the lipid sensors could be partly involved in the dysfunction of glucose homeostasis. The study aimed at comparing the effect of Medialipid (ML) overload on insulin secretion and sensitivity when administered either through the intestine or the carotid artery in mice. An indwelling intragastric or intracarotid catheter was installed in mice and ML or an isocaloric solution was infused over 24 hours. Glucose and insulin tolerance and vagus nerve activity were assessed. Some mice were treated daily for one week with the anti-lipid peroxidation agent aminoguanidine prior to the infusions and tests. The intestinal but not the intracarotid infusion of ML led to glucose and insulin intolerance when compared with controls. The intestinal ML overload induced lipid accumulation and increased lipid peroxidation as assessed by increased malondialdehyde production within both jejunum and duodenum. These effects were associated with the concomitant deregulation of vagus nerve. Administration of aminoguanidine protected against the effects of lipid overload and normalized glucose homeostasis and vagus nerve activity. Lipid overload within the intestine led to deregulation of gastrointestinal lipid sensing that in turn impaired glucose homeostasis through changes in autonomic nervous system activity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Paraformaldehyde, powder, 95%
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
Sigma-Aldrich
Paraformaldehyde, reagent grade, crystalline
Sigma-Aldrich
Glutaraldehyde solution, Grade II, 25% in H2O
Sigma-Aldrich
1-Butanol, anhydrous, 99.8%
Sigma-Aldrich
Glutaraldehyde solution, 50 wt. % in H2O
Sigma-Aldrich
1-Butanol, 99.9%
Sigma-Aldrich
1-Butanol, for molecular biology, ≥99%
Sigma-Aldrich
1-Butanol, ACS reagent, ≥99.4%
Sigma-Aldrich
3,3′-Diaminobenzidine, ≥97% (HPLC)
Sigma-Aldrich
1-Butanol, suitable for HPLC, ≥99.7%
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 50% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Paraformaldehyde, prilled, 95%
Sigma-Aldrich
3,3′-Diaminobenzidine, 98%
Sigma-Aldrich
1-Butanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 70% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Supelco
1-Butanol, analytical standard
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 8% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Glutaraldehyde solution, 50% in H2O, suitable for photographic applications
Sigma-Aldrich
Ile-Pro-Ile, ≥97% (HPLC)
Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%
Supelco
Chloroform solution, certified reference material, 5000 μg/mL in methanol
Sigma-Aldrich
Chloroform solution, NMR reference standard, 3% in acetone-d6 (99.9 atom % D)
Supelco
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 5 mm × 8 in.
Sigma-Aldrich
1-Butanol, BioRenewable, ACS reagent, ≥99.4%
Supelco
Chloroform solution, 200 μg/mL in methanol, analytical standard
Supelco
Chloroform solution, NMR reference standard, 1% in acetone-d6 (99.9 atom % D)
Sigma-Aldrich
Chloroform solution, NMR reference standard, 10% in acetone-d6 (99.9 atom % D), NMR tube size 3 mm × 8 in.
Supelco
Chloroform solution, NMR reference standard, 1% in acetone-d6 (99.9 atom % D), NMR tube size 8 mm × 8 in.
Supelco
Chloroform solution, NMR reference standard, 5% in acetone-d6 (99.9 atom % D), NMR tube size 5 mm × 8 in.

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.