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Robust production of a peptide library using methodological synchronization.

Protein expression and purification (2009-05-22)
Kristen L Huber, Kevin D Olson, Jeanne A Hardy

Peptide libraries have proven to be useful in applications such as substrate profiling, drug candidate screening and identifying protein-protein interaction partners. However, issues of fidelity, peptide length, and purity have been encountered when peptide libraries are chemically synthesized. Biochemically produced libraries, on the other hand, circumvent many of these issues due to the fidelity of the protein synthesis machinery. Using thioredoxin as an expression partner, a stably folded peptide scaffold (avian pancreatic polypeptide) and a compatible cleavage site for human rhinovirus 3C protease, we report a method that allows robust expression of a genetically encoded peptide library, which yields peptides of high purity. In addition, we report the use of methodological synchronization, an experimental design created for the production of a library, from initial cloning to peptide characterization, within a 5-week period of time. Total peptide yields ranged from 0.8% to 16%, which corresponds to 2-70 mg of pure peptide. Additionally, no correlation was observed between the ability to be expressed or overall yield of peptide-fusions and the intrinsic chemical characteristics of the peptides, indicating that this system can be used for a wide variety of peptide sequences with a range of chemical characteristics.

Product Number
Product Description

Brilliant Blue G, pure
Ampicillin, anhydrous, 96.0-102.0% (anhydrous basis)
Brilliant Blue G, 250, for microscopy
Ampicillin, Ready Made Solution, 100 mg/mL, 0.2 μm filtered
Ampicillin, meets USP testing specifications
Brilliant Blue R, Dye content ~50 %, Technical grade
Brilliant Blue R, pure
Brilliant Blue R, 250, for microscopy