MilliporeSigma

Simple method of specimen preparation for scanning electron microscopy.

Bulletin of experimental biology and medicine (2012-03-28)
S V Buravkov, V P Chernikov, L B Buravkova
ABSTRACT

We compared conventional method for specimen (cell cultures, tissue specimens) preparation for scanning electron microscopy and a method without sputtering and critical-point drying. OTO-method (osmium-thiocarbohydrazide-osmium) with sample impregnation with hexamethyldisilazane followed by air drying was used as an alternative method. Excellent preservation of surface ultrastructures and electrical conductivity was proved. The method is easy to use and does not require additional costs for equipment.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
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Supelco
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