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Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes.

Hybridoma (1984-01-01)
C B Reimer, D J Phillips, C H Aloisio, D D Moore, G G Galland, T W Wells, C M Black, J S McDougal
ABSTRACT

Stable clones of 31 mouse hybridomas that produce monoclonal antibodies (MAbs) against human IgG antigenic determinants were obtained. The number of hybridomas of different specificity described are: 2 anti-IgG1 Fc, 1 anti-IgG2 Fc, 1 anti-IgG2 Fd, 2 anti-IgG3 Fc, 2 anti-IgG3 hinge, 3 anti-IgG4 Fc, 3 anti-IgG4 Fd, 2 anti-nG4m(b), 4 anti-IgGFc, 2 anti-IgGFd, 1 anti-kappa, 1 anti-lambda, 1 anti-non IgG1, 2 anti-non IgG2, 2 anti-non-IgG3, 2 anti-non-IgG4. Evidence is presented validating their specificity. Some MAbs demonstrated to be avid, potent, and specific for well defined IgG-subclass epitopes may be partially or completely inactive in other assay systems, presumably because of different presentations of antigen epitopes. In general, this problem requires careful writing of protocols describing the use of MAbs.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-Human IgG4−FITC antibody produced in mouse, clone HP-6025, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-Human IgG (Fc specific)−Biotin antibody, Mouse monoclonal, clone HP-6017, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Human IgG2−FITC antibody produced in mouse, clone HP-6014, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse, clone HP-6017, ascites fluid
Sigma-Aldrich
Monoclonal Anti-Human IgG1 (Fc specific) antibody produced in mouse, clone HP-6001, ascites fluid
Sigma-Aldrich
Anti-Human IgG4−Biotin antibody, Mouse monoclonal, clone HP-6025, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Human IgG4 antibody produced in mouse, clone HP-6025, ascites fluid
Sigma-Aldrich
Anti-Human IgG (γ-chain specific) antibody, Mouse monoclonal, clone GG-5, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse, clone GG-7, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Human IgG2 antibody produced in mouse, clone HP-6014, ascites fluid
Sigma-Aldrich
Anti-Human IgG (Fc specific)-Peroxidase antibody, Mouse monoclonal, clone HP-6017, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Human IgG2 (Fc specific) antibody produced in mouse, clone HP-6002, ascites fluid
Sigma-Aldrich
Monoclonal Anti-Human IgG (Fc specific)−FITC antibody produced in mouse, clone HP-6017, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse, clone HP-6054, ascites fluid
Sigma-Aldrich
Anti-Human IgG1 (Fc specific) antibody, Mouse monoclonal, clone HP-6001, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal, clone GG-6, purified from hybridoma cell culture
Sigma-Aldrich
Mouse Anti-Human IgG1 Antibody, clone HP6001, Fc, clone HP6001, Chemicon®, from mouse
Sigma-Aldrich
Anti-Human IgG4-Peroxidase antibody, Mouse monoclonal, clone HP-6025, purified from hybridoma cell culture
Sigma-Aldrich
Mouse Anti-Human IgG4 Antibody, clone HP6023, Fc, clone HP6023, Chemicon®, from mouse
Sigma-Aldrich
Anti-Human IgG (Fc specific)-Peroxidase antibody, Mouse monoclonal, clone GG-7, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Human IgG3 antibody, Mouse monoclonal, clone HP-6050, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Human IgG3-Peroxidase antibody, Mouse monoclonal, clone HP-6050, purified from hybridoma cell culture
HP 6053, 94050510, Reactivity: Anti-κ-Chain, human
Sigma-Aldrich
Anti-Human IgG2 antibody, Mouse monoclonal, clone HP-6014, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Human IgG4 antibody produced in mouse, clone HP-6025, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Human IgG2−Biotin antibody produced in mouse, clone HP-6014, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-Human IgG4 (pFc′ specific) antibody produced in mouse, clone HP-6023, ascites fluid