Human IgG subclass measurements in the clinical laboratory.

Clinical chemistry (1987-10-01)
R G Hamilton
ABSTRACT

Complement activation, cell surface-receptor binding, blocking activity, and possibly placental transfer are among the biologically important functional differences that have been detected between the four human IgG subclasses by use of polyclonal antisera. In 1985, a IUIS/WHO panel of immunologists, using eight immunological methods, documented the specificity of select monoclonal antibodies for the IgG subclasses. Clinical assays have been developed involving these monoclonal antibodies that allow quantification of the concentration of IgG subclass protein and distribution of the IgG subclass antibodies in human immune responses. This review addresses issues of concern to investigators who are evaluating and (or) developing quantitative human IgG subclass assays in the clinical laboratory. Unique physical (structural) and biological (functional) properties of human IgG subclasses are summarized, with a focus on aspects pertinent to their clinical importance and in vitro quantification. The HP-series monoclonal antibodies with documented specificity are examined within the context of their application to several immunological methods. I describe unique technical aspects of total and antigen-specific IgG-subclass immunoassays involving these monoclonal antibodies. Finally, this report outlines clinical applications and indications for IgG-subclass measurements in the study of human health and disease.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Mouse IgG (Fc specific) antibody produced in goat, 2.0 mg/mL, affinity isolated antibody
Sigma-Aldrich
IgG from human serum, technical grade, ≥80% (SDS-PAGE), buffered aqueous solution
Sigma-Aldrich
Anti-Rabbit IgG (whole molecule)–Gold antibody produced in goat, affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)
Sigma-Aldrich
Monoclonal Anti-Human IgG4−FITC antibody produced in mouse, clone HP-6025, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-Human IgG3−FITC antibody produced in mouse, clone HP-6050, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Human IgG1−FITC antibody produced in mouse, clone 8c/6-39, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-Human IgG2−FITC antibody produced in mouse, clone HP-6014, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-Human IgG (whole molecule)−FITC antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
Anti-Human IgG4−Biotin antibody, Mouse monoclonal, clone HP-6025, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Human IgG4 antibody produced in mouse, clone HP-6025, ascites fluid
Sigma-Aldrich
Anti-Human IgG (Fc specific), F(ab′)2 fragment antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Cat IgG (whole molecule)−FITC antibody produced in goat, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-Human IgG1−Biotin antibody produced in mouse, clone 8c/6-39, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-Human IgG3 antibody produced in mouse, clone HP-6050, ascites fluid
Sigma-Aldrich
Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Human IgG (γ-chain specific)-Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-Human IgG2 antibody produced in mouse, clone HP-6014, ascites fluid
Sigma-Aldrich
Monoclonal Anti-Human IgG2 (Fc specific) antibody produced in mouse, clone HP-6002, ascites fluid
Sigma-Aldrich
Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Peroxidase antibody produced in goat, affinity isolated antibody
Sigma-Aldrich
Anti-Rabbit IgG (whole molecule) antibody produced in goat, whole antiserum, lyophilized powder
Sigma-Aldrich
Anti-Mouse IgG (whole molecule)–R-Phycoerythrin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-Human IgG3−Biotin antibody produced in mouse, clone HP-6050, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
IgG2b, Kappa from murine myeloma, clone MOPC 195, ascites fluid, lyophilized powder
Sigma-Aldrich
Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) Biotin Conjugate, liquid, clone HP6069, Calbiochem®
Sigma-Aldrich
Monoclonal Anti-Human IgG4 antibody produced in mouse, clone HP-6025, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Human IgG1 antibody produced in mouse, clone 8c/6-39, ascites fluid
Sigma-Aldrich
Monoclonal Anti-Human IgG2−Biotin antibody produced in mouse, clone HP-6014, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−FITC antibody produced in goat, affinity isolated antibody, buffered aqueous solution

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.