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Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic Acid-conjugated dendrimers.

ACS nano (2009-02-12)
Kevin J Landmark, Stassi Dimaggio, Jesse Ward, Christopher Kelly, Stefan Vogt, Seungpyo Hong, Alina Kotlyar, Andrzej Myc, Thommey P Thomas, James E Penner-Hahn, James R Baker, Mark M Banaszak Holl, Bradford G Orr
ABSTRACT

Organic-coated superparamagnetic iron oxide nanoparticles (OC-SPIONs) were synthesized and characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. OC-SPIONs were transferred from organic media into water using poly(amidoamine) dendrimers modified with 6-TAMRA fluorescent dye and folic acid molecules. The saturation magnetization of the resulting dendrimer-coated SPIONs (DC-SPIONs) was determined, using a superconducting quantum interference device, to be 60 emu/g Fe versus 90 emu/g Fe for bulk magnetite. Selective targeting of the DC-SPIONs to KB cancer cells in vitro was demonstrated and quantified using two distinct and complementary imaging modalities: UV-visible and X-ray fluorescence; confocal microscopy confirmed internalization. The results were consistent between the uptake distribution quantified by flow cytometry using 6-TAMRA UV-visible fluorescence intensity and the cellular iron content determined using X-ray fluorescence microscopy.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
6-Carboxytetramethylrhodamine, BioReagent, suitable for fluorescence, ≥90% (HPLC)

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