• Home
  • Search Results
  • Formation of arenicin-1 microdomains in bilayers and their specific lipid interaction revealed by Z-scan FCS.

Formation of arenicin-1 microdomains in bilayers and their specific lipid interaction revealed by Z-scan FCS.

Analytical and bioanalytical chemistry (2011-02-05)
Radek Macháň, Martin Hof, Tatsiana Chernovets, Maxim N Zhmak, Tatiana V Ovchinnikova, Jan Sýkora
ABSTRACT

Z-scan fluorescence correlation spectroscopy (FCS) is employed to characterize the interaction between arenicin-1 and supported lipid bilayers (SLBs) of different compositions. Lipid analogue C8-BODIPY 500/510C5-HPC and ATTO 465 labelled arenicin-1 are used to detect changes in lipid and peptide diffusion upon addition of unlabelled arenicin-1 to SLBs. Arenicin-1 decreases lipid mobility in negatively charged SLBs. According to diffusion law analysis, microdomains of significantly lower lipid mobility are formed. The analysis of peptide FCS data confirms the presence of microdomains for anionic SLBs. No indications of microdomain formation are detected in SLBs composed purely of zwitterionic lipids. Additionally, our FCS results imply that arenicin-1 exists in the form of oligomers and/or aggregates when interacting with membranes of both compositions.

MATERIALS
Product Number
Brand
Product Description

SAFC
Atto 465 NHS ester, BioReagent, suitable for fluorescence, ≥80% (HPCE)
Sigma-Aldrich
Atto 465 maleimide, BioReagent, suitable for fluorescence, ≥90% (coupling to thiols)

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.