Analysis of age-related global DNA methylation in chicken.

Biochemical genetics (2013-04-05)
Magdalena Gryzinska, Ewa Blaszczak, Aneta Strachecka, Grazyna Jezewska-Witkowska
ABSTRACT

DNA methylation is an epigenetic modification that plays an important role in the normal development and function of organisms. The level of DNA methylation is species-, tissue-, and organelle-specific, and the methylation pattern is determined during embryogenesis. DNA methylation has also been correlated with age. The aim of this study was to determine the global DNA methylation levels and their correlation with age in the chicken, using a Polish autosexing chicken breed, Polbar. A quantitative technique based on an immunoenzymatic assay was used for global DNA methylation analysis. The results show increased global DNA methylation levels with older Polbar embryos. Global DNA methylation levels decrease with the age of hens in the postembryonic stage. This study expands the current knowledge of the Polbar epigenome and the general knowledge of the function of epigenetic mechanisms in birds.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Imprint® Methylated DNA Quantification Kit, To measure global DNA methylation shifts from as low as 10 ng DNA