DNA transfection: calcium phosphate method.

Methods in molecular biology (Clifton, N.J.) (2013-05-18)
Munjin Kwon, Bonnie L Firestein
ABSTRACT

The calcium phosphate transfection is a widely used method for introducing foreign DNA plasmids into cells. Mechanisms underlying this transfection method are not yet defined; however, DNA-calcium phosphate precipitates are internalized by the cells and DNA is efficiently expressed in almost all cell types. The cost-efficiency and simplicity of this method allows for use in primary neuronal cultures, despite issues of neurotoxicity. Here, we describe an optimized calcium phosphate transfection method for the delivery of DNA plasmid into primary dissociated neuronal cultures.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Calcium Phosphate Transfection Kit, Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells