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A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases.

Acta biochimica Polonica (2012-11-07)
Beata Podgórska, Grażyna Kujawska, Michał Skurzewski, Olesya Batsko, Tadeusz Kaczorowski
ABSTRACT

In this work we describe a novel, rapid and simple microscale procedure for identification of restriction endonuclease activity in bacteria lysates, which contain high levels of non-specific DNA nucleases.