• Home
  • Search Results
  • Stabilization of RNA through absorption by functionalized mesoporous silicate nanospheres.

Stabilization of RNA through absorption by functionalized mesoporous silicate nanospheres.

PloS one (2012-12-12)
Brandy J Johnson, Brian J Melde, Michael A Dinderman, Baochuan Lin
ABSTRACT

The potential for encapsulating RNA within tunable, semi-permeable structures for storage and transportation purposes offers an interesting approach to the reduction of stringent storage requirements that often hamper the field application of genetic analysis methods. In this study, we assessed the potential for application of functionalized, porous silicate sorbents in maintaining nucleic acid integrity. Mesoporous silica nanoparticles (MSNs) with and without incorporated stabilizing reagents were used to encapsulate triosephosphate isomerase mRNA of Arabidopsis thaliana. The absorption, elution, and the long-term stability of the RNA were monitored by using quantitative real-time RT-PCR. The results indicate that adsorbed RNA can be eluted from the sorbents using simple buffers and employed directly for downstream molecular diagnostic assays without any further processing. RNA integrity can be maintained for extended time periods under refrigeration temperatures in the presence of covalently immobilized stabilizing compounds. This study provides initial evidence of the potential for application of MSNs in transportation and storage. They may also have utility in sample collection and processing in restrictive environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Hexadecyltrimethylammonium bromide, ≥98%
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, for molecular biology, ≥99%
Sigma-Aldrich
Cetyltrimethylammonium chloride solution, 25 wt. % in H2O
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, BioXtra, ≥99%
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, BioUltra, for molecular biology, ≥99.0% (AT)
Sigma-Aldrich
Hexadecyltrimethylammonium chloride, ≥98.0% (NT)
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, ≥96.0% (AT)
Sigma-Aldrich
Calcium silicate, purum, 12-22% Ca (as CaO) basis, ≥87% SiO2 basis
Sigma-Aldrich
Hexadecyltrimethylammonium hydroxide solution, 10 wt. % in H2O
Sigma-Aldrich
Calcium silicate, −200 mesh, 99%
Supelco
Hexadecyltrimethylammonium bromide, suitable for ion pair chromatography, LiChropur
Sigma-Aldrich
Triosephosphate Isomerase from rabbit muscle, Type X, lyophilized powder, ≥3,500 units/mg protein
Sigma-Aldrich
Hexadecyltrimethylammonium hydroxide solution, ~25% in methanol (T)
Supelco
Hexadecyltrimethylammonium bromide, analytical standard
Sigma-Aldrich
Triosephosphate Isomerase from rabbit muscle, Type III-S, ammonium sulfate suspension, ≥4,000 units/mg protein
Sigma-Aldrich
Cetyltrimethylammonium hydrogensulfate, 99%
Sigma-Aldrich
Triosephosphate Isomerase from baker's yeast (S. cerevisiae), Type I, ammonium sulfate suspension, ~10,000 units/mg protein
Supelco
Hexadecyltrimethylammonium bisulfate, suitable for ion pair chromatography, LiChropur, ≥99.0% (T)

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.