Defective G-CSFR signaling pathways in congenital neutropenia.

Hematology/oncology clinics of North America (2013-01-29)
Julia Skokowa, Karl Welte
ABSTRACT

Several signaling systems downstream of G-CSFR have been identified that are defective or hyperactivated in myeloid cells of patients with congenital neutropenia: severely reduced expression of myeloid-specific transcription factors LEF-1 and C/EBPα, severely reduced expression and functions of HCLS1 protein, severely reduced expression of neutrophil elastase protein, dramatic compensatory up-regulation of the NAMPT/NAD(+)/SIRT pathway leading to continuous activation of emergency granulopoiesis via the transcription factor C/EBPβ, and hyperactivation of STAT5 protein by tyrosine phosphorylation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Elastase from human leukocytes, lyophilized powder, ≥50 units/mg protein (Bradford)