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Immunomodulatory activity of oenothein B isolated from Epilobium angustifolium.

Journal of immunology (Baltimore, Md. : 1950) (2009-10-23)
Igor A Schepetkin, Liliya N Kirpotina, Larissa Jakiw, Andrei I Khlebnikov, Christie L Blaskovich, Mark A Jutila, Mark T Quinn
ABSTRACT

Epilobium angustifolium has been traditionally used to treat of a number of diseases; however, not much is known regarding its effect on innate immune cells. In this study, we report that extracts of E. angustifolium activated functional responses in neutrophils and monocyte/macrophages. Activity-guided fractionation, followed by mass spectroscopy and NMR analysis, resulted in the identification of oenothein B as the primary component responsible for phagocyte activation. Oenothein B, a dimeric hydrolysable tannin, dose-dependently induced a number of phagocyte functions in vitro, including intracellular Ca(2+) flux, production of reactive oxygen species, chemotaxis, NF-kappaB activation, and proinflammatory cytokine production. Furthermore, oenothein B was active in vivo, inducing keratinocyte chemoattractant production and neutrophil recruitment to the peritoneum after intraperitoneal administration. Biological activity required the full oenothein B structure, as substructures of oenothein B (pyrocatechol, gallic acid, pyrogallol, 3,4-dihydroxybenzoic acid) were all inactive. The ability of oenothein B to modulate phagocyte functions in vitro and in vivo suggests that this compound is responsible for at least part of the therapeutic properties of E. angustifolium extracts.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Zymosan A from Saccharomyces cerevisiae, for inducing inflamatory response
Supelco
Oenothein B, analytical standard