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Cloning and recombinant expression of phospholipase A2 present in rheumatoid arthritic synovial fluid.

The Journal of biological chemistry (1989-04-05)
J J Seilhamer, W Pruzanski, P Vadas, S Plant, J A Miller, J Kloss, L K Johnson
ABSTRACT

Synovial fluid from arthritic patients contains multiple forms of phospholipase A2 (PLA2), as resolved by high performance liquid chromatography (Seilhamer, J.J., Plant, S., Pruzanski, W., Schilling, J., Stefanski, E., Vadas, P., and Johnson, L. K. (1989) J. Biochem. (Tokyo), submitted for publication). Here we describe the cloning of a human 4.5-kilobase gene and 800-base pair cDNA encoding the form representing the major peak of activity and protein mass (peak A). The clones encode a mature peptide of 124 amino acids, which follows a prepeptide of 20 residues. The deduced amino acid sequence constitutes an enzyme of the "Type II" class of PLA2s, and resembles PLA2s from other mammalian sources. This represents the first report of a full length mammalian non-pancreatic PLA2 sequence. Active transcription of this PLA2 gene was detected in two different inflammatory cell sources. Recombinant human peak A PLA2 was expressed in vaccinia as a secreted protein which accumulated in conditioned medium.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phospholipase A2 from honey bee venom (Apis mellifera), salt-free, lyophilized powder, 600-2400 units/mg protein
Sigma-Aldrich
Phospholipase A2 from bovine pancreas, lyophilized powder, ≥20 units/mg protein
Sigma-Aldrich
Phospholipase A2 from porcine pancreas, ammonium sulfate suspension, ≥600 units/mg protein

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