The human myocardial two-dimensional gel protein database: update 1994.

Electrophoresis (1994-11-01)
J M Corbett, C H Wheeler, C S Baker, M H Yacoub, M J Dunn
ABSTRACT

An updated human heart protein two-dimensional electrophoresis (2-DE) database is presented. The database, which contains some 1388 protein spots characterised in terms of M(r) and pI, has been analysed further by Western immunoblotting and protein sequencing. From a total of 103 protein spots analysed, 49 have been identified by immunoblotting and 32 have been identified by protein sequencing. A further six proteins have tentatively been assigned by comparison with the human heart 2-DE protein database of Jungblut et al. (Electrophoresis) 1994, 15, 685-607). This database is being used in studies of alterations in protein expression in the diseased and transplanted human heart.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Malic Dehydrogenase from porcine heart, ≥600 units/mg protein (biuret), ammonium sulfate suspension
Sigma-Aldrich
Malic Dehydrogenase from porcine heart, buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)
Sigma-Aldrich
Malic Dehydrogenase from bovine heart, ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)
Sigma-Aldrich
Malic Dehydrogenase from porcine heart, ≥400 units/mg protein (biuret), ammonium sulfate suspension