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Cloning, functional expression and characterization of a human olfactory receptor.

Cellular and molecular biology (Noisy-le-Grand, France) (1999-07-01)
H Hatt, G Gisselmann, C H Wetzel
ABSTRACT

The human olfactory system can recognize and discriminate a large number of different odorant molecules. The detection of chemically distinct odorants begins with the binding of an odorant ligand to a specific receptor protein on the olfactory neuron cell surface. To address the problem of olfactory perception at a molecular level, we have cloned, functionally expressed and characterized the first human olfactory receptor (OR 17-40). Application of a mixture of hundred different odorants elicited a transient increase in intracellular calcium at HEK 293-cells which were transfected with a plasmid containing the receptor encoding DNA and a membrane import sequence. By subdividing the odorant mixture in smaller groups we could identify a single component which represented the only effective substance: helional. Testing some structurally closely related molecules we found only one other compound which also could activate the receptor: heliotropyl acetone. All other compounds tested were completely ineffective. These findings represent the beginning of molecular understanding of odorant recognition in humans.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Piperonal, 99%
Sigma-Aldrich
Piperonal, ≥99%, FCC, FG
Sigma-Aldrich
Piperonal, natural, 98%, FG