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Method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines.

Journal of agricultural and food chemistry (1999-12-20)
V Fogliano, V Verde, G Randazzo, A Ritieni
ABSTRACT

A novel method for measuring the antioxidant activity using N, N-dimethyl-p-phenylenediamine (DMPD) was developed. The radical cation of this compound gives a stable colored solution and a linear inhibition of color formation can be observed in the presence of 0. 2-11 microg of TROLOX. The experimental protocol, which is rapid and inexpensive, ensures sensitivity and reproducibility in the measure of antioxidant activity of hydrophilic compounds. The effectiveness of the DMPD method on real foods was verified by evaluating the antioxidant ability of wine samples coming from different areas of Campania, Italy. Antioxidant capacity of wines is strictly related to the amount of phenolic compounds. The results obtained by the DMPD method are very similar to those obtained on the same samples when the radical cation of 2, 2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (Miller et al., 1996) was used.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
N,N-Dimethyl-p-phenylenediamine dihydrochloride, suitable for peroxidase test, ≥99.0% (titration)
Sigma-Aldrich
N,N-Dimethyl-p-phenylenediamine, 97%
Millipore
N,N-Dimethyl-p-phenylenediamine dihydrochloride, suitable for microbiology, ≥99.0%
Sigma-Aldrich
N,N-Dimethyl-1,4-phenylenediamine oxalate, 98%
Supelco
N,N-Dimethyl-p-phenylenediamine, for spectrophotometric det. of SO42-, S2-, ≥97.0%
Sigma-Aldrich
N,N-Dimethyl-p-phenylenediamine sulfate salt, 98%