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Ferrocenecarboxaldehyde labeled DNA probe for the study on DNA damage and protection.

Fresenius' journal of analytical chemistry (2001-02-28)
C Xu, H Cai, P He, Y Fang
ABSTRACT

A ferrocenecarboxaldehyde (FCA) labeled DNA probe is used for the first time in the study of DNA damage and protection. The electrochemically active reagent FCA was labeled successfully on to a denatured calf-thymus DNA by 1-ethyl-3- (3-dimethyl-aminopropyl) carbodiimide (EDC). The FCA labeled DNA probe was used to hybridize with the sample DNA sequence accumulated on the surface of a graphite electrode. The anodic peaks of the FCA bound to the double-stranded DNA (dsDNA) by differential pulse voltammetry (DPV) were used for the detection of DNA damage and protection. Thiourea, sodium benzoic acid and isopropanol can decrease DNA damage by hydroxyl radicals, and their protection efficiencies are discussed.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ferrocenecarboxaldehyde, 98%
Supelco
Ferrocenecarboxaldehyde, for HPLC derivatisation, LiChropur, ≥98.0% (HPLC)

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