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Identification of a 2,6-dichloroisonicotinic-acid-sensitive protein kinase from tobacco by affinity chromatography on benzothiadiazole-sepharose and NIM-metal chelate adsorbent.

Pest management science (2001-08-24)
C Pillonel
ABSTRACT

In the search for the target site of inducers of systemic acquired resistance (SAR), a BTH-binding protein kinase (BBPK) has been identified from tobacco by affinity chromatography on benzothiadiazole-sepharose (CGA 324041-sepharose) and NIM-metal chelate affinity resin. The substrate selectivity of the isolated enzyme (phosphorylation of histone type III-S, I kappa B alpha,IkB alpha S32A/S36A and NIM1) suggested a possible BBPK-mediated regulation of NIM1 in tobacco. The measurement of the effect of different SAR-inducers showed an inhibition of BBPK by 2,6-dichloroisonicotinic acid (INA) and, to a lower extent, by benzothiadiazoles and salicylic acid. Comparison between BBPK cell-free inhibition and in vivo PR-1 induction revealed that BBPK could be the target site of INA.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2,6-Dichloropyridine-4-carboxylic acid, 98%