Skip to Content
MilliporeSigma
  • Role of kallikrein-kininogen system in insulin-stimulated glucose transport after muscle contractions.

Role of kallikrein-kininogen system in insulin-stimulated glucose transport after muscle contractions.

Journal of applied physiology (Bethesda, Md. : 1985) (2002-01-18)
C L Dumke, J Kim, E B Arias, G D Cartee
ABSTRACT

Serum proteins [molecular weight (MW) > 10,000] are essential for increased insulin-stimulated glucose transport after in vitro muscle contractions. We investigated the role of the kallikrein-kininogen system, including bradykinin, which is derived from kallikrein (MW > 10,000)-catalyzed degradation of serum protein kininogen (MW > 10,000), on this contraction effect. In vitro electrical stimulation of rat epitrochlearis muscles was performed in 1) rat serum +/- kallikrein inhibitors; 2) human plasma (normal or kallikrein-deficient); 3) rat serum +/- bradykinin receptor-2 inhibitors; or 4) serum-free buffer +/- bradykinin. 3-O-methylglucose transport (3-MGT) was measured 3.5 h later. Serum +/- kallikrein inhibitors tended (P = 0.08) to diminish postcontraction insulin-stimulated 3-MGT. Contractions in normal plasma enhanced insulin-stimulated 3-MGT vs. controls, but contractions in kallikrein-deficient plasma did not. Supplementing rat serum with bradykinin receptor antagonist HOE-140 during contraction did not alter insulin-stimulated 3-MGT. Muscles stimulated to contract in serum-free buffer plus bradykinin did not have enhanced insulin-stimulated 3-MGT. Bradykinin was insufficient for postcontraction-enhanced insulin sensitivity. However, results with kallikrein inhibitors and kallikrein-deficient plasma suggest kallikrein plays a role in this improved insulin action.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Trypsin inhibitor from chicken egg white, Type III-O (free of ovoinhibitor)
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), solution, sterile-filtered, suitable for, suitable for cell culture
Sigma-Aldrich
Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), For use as a marker in SDS-PAGE, BioReagent
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), lyophilized powder
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), lyophilized powder
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), Isoelectric focusing marker, pI 4.6
Sigma-Aldrich
Trypsin Inhibitor, Defined (1X) Solution, Animal component free, BioReagent, suitable for cell culture
Sigma-Aldrich
Trypsin inhibitor from chicken egg white, Type II-O, Partially purified ovomucoid, containing ovoinhibitor
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), lyophilized powder, ~10000 U/mg