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Utilization of methoxylated benzoates and formation of intermediates by Desulfotomaculum thermobenzoicum in the presence or absence of sulfate.

Archives of microbiology (1992-01-01)
M Tasaki, Y Kamagata, K Nakamura, E Mikami
ABSTRACT

Desulfotomaculum thermobenzoicum strain TSB (DSM 6193) was found to utilize some methoxylated benzoates as carbon and energy source with or without sulfate. 3- or 4-Methoxybenzoate, vanillate (4-hydroxy-3-methoxybenzoate), syringate (3,5-dimethoxy-4-hydroxybenzoate) and 3,4,5-trimethoxybenzoate were converted to corresponding hydroxybenzoates. However, neither 2-methoxybenzoate nor 2,6-dimethoxybenzoate was utilized. The organism grew acetogenically on each of the methoxylated benzoates in the absence of sulfate. 3,4-Dihydroxy-5-methoxybenzoate was detected during conversion of syringate, and syringate and 3,4-dihydroxy-5-methoxybenzoate were detected during conversion of 3,4,5-trimethoxybenzoate as intermediates. These findings indicate that 4-methoxyl-group is most readily cleaved, whereas 2-methoxyl-group is not utilized by the organism.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
4-Methoxybenzoic acid, ReagentPlus®, 99%
Sigma-Aldrich
3-Methoxybenzoic acid, ReagentPlus®, 99%
Sigma-Aldrich
3,4,5-Trimethoxybenzoic acid, ReagentPlus®, 99%
Supelco
Melting point standard 182-184°C, analytical standard
Sigma-Aldrich
p-Anisic acid, ≥99%, FG
Sigma-Aldrich
p-Anisic acid, natural, 99%, FG
Sigma-Aldrich
m-Anisic acid, ≥98%