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[Determination of isothiocyanates in human urine by high performance liquid chromatography].

Se pu = Chinese journal of chromatography (2005-02-17)
Xingdi Chu, Lifang Zhu, Yutang Gao

A reversed-phase high performance liquid chromatographic method (HPLC) was established for determination of isothiocyanates in human urine, which used the reaction of propyl isothiocyanate with 1,2-benzenedithiol as reference instead of the synthesis of 1,3-benzodithiole-2-thione. The linear regression of the standard curve was determined to be y = 0.418 2 x + 2.821 (r2 = 0.999 3), that was well matched with the regression of methyl isothiocyanate, y = 0.412 2x + 2.442 3(r2 = 0.996 6). The detection limit (at approximately S/N = 2.5) was 0.08 micromol/L. The mean relative standard deviation (RSD) (n = 21) for the intra-day reproducibility was (4.7 +/- 2.9)%. The mean RSD (n = 4) for inter-day reproducibility of three samples was (7.0 +/- 5.0)%. The recovery of four propyl isothiocyanate standards was (91.3 +/- 7.9)% (mean RSD 8.7%). The initial results obtained by this method may provide useful information regarding to the kinetics of isothiocyanates in dietary consumption and excretion of individuals.

Product Number
Product Description

Methyl isothiocyanate, 97%
Methyl isothiocyanate, PESTANAL®, analytical standard

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