• Home
  • Search Results
  • [Determination of isothiocyanates in human urine by high performance liquid chromatography].

[Determination of isothiocyanates in human urine by high performance liquid chromatography].

Se pu = Chinese journal of chromatography (2005-02-17)
Xingdi Chu, Lifang Zhu, Yutang Gao
ABSTRACT

A reversed-phase high performance liquid chromatographic method (HPLC) was established for determination of isothiocyanates in human urine, which used the reaction of propyl isothiocyanate with 1,2-benzenedithiol as reference instead of the synthesis of 1,3-benzodithiole-2-thione. The linear regression of the standard curve was determined to be y = 0.418 2 x + 2.821 (r2 = 0.999 3), that was well matched with the regression of methyl isothiocyanate, y = 0.412 2x + 2.442 3(r2 = 0.996 6). The detection limit (at approximately S/N = 2.5) was 0.08 micromol/L. The mean relative standard deviation (RSD) (n = 21) for the intra-day reproducibility was (4.7 +/- 2.9)%. The mean RSD (n = 4) for inter-day reproducibility of three samples was (7.0 +/- 5.0)%. The recovery of four propyl isothiocyanate standards was (91.3 +/- 7.9)% (mean RSD 8.7%). The initial results obtained by this method may provide useful information regarding to the kinetics of isothiocyanates in dietary consumption and excretion of individuals.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methyl isothiocyanate, 97%
Supelco
Methyl isothiocyanate, PESTANAL®, analytical standard

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.