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Cumene peroxide and Fe(2+)-ascorbate-induced lipid peroxidation and effect of phosphoglucose isomerase.

Molecular and cellular biochemistry (2006-04-04)
Z S Agadjanyan, S F Dugin, L F Dmitriev
ABSTRACT

Malondialdehyde (MDA) is one of cytotoxic aldehydes produced in cells as a result of lipid peroxidation and further MDA metabolism in cytoplasm is not known. In our experiments the liver fraction 10,000 g containing phosphoglucose isomerase and enzymes of the glyoxalase system was used and obtained experimental data shows that in this fraction there is an aggregate of reactions taking place both in membranes (lipid peroxidation) and outside membranes. MDA accumulation is relatively slow because MDA is a substrate of aldehyde isomerase (MDA <--> methylglyoxal). The well known enzyme phosphoglucose isomerase acts as an aldehyde isomerase (Michaelis constant for this enzyme Km = 133 +/- 8 microM). MDA conversion to methylglyoxal and further to neutral product D-lactate (with GSH as a cofactor) occurs in cytoplasm and D-lactate should be regarded as the end product of two different parametabolic reactions: lipid peroxidation or protein glycation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Cumene, 98%
Supelco
Cumene, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Cumene, PESTANAL®
Supelco
Cumene solution, certified reference material, 5000 μg/mL in methanol