Analysis of micronuclei, nuclear buds, bi-polynucleated and fragmented-apoptotic cells was performed in gills of blue mussels exposed for 3 weeks to sublethal concentrations of bisphenol A, diallyl phthalate (for the both nominal concentration 50 ppb) and to tetrabromodiphenyl ether-47 (nominal concentration 5 ppb). Fourteen specimens from each treatment and control group were used for the analysis. Our results demonstrated a significant increase in micronuclei frequency after the treatment with bisphenol A (P=0.0243), diallyl phthalate (P=0.0005) and tetrabromodiphenyl ether-47 (P<0.0001; Mann-Whitney U-test). Induction of bi-nucleated (P=0.0028), fragmented-apoptotic (P=0.0004) cells and nuclear buds (P=0.0101) was found in mussels exposed to tetrabromodiphenyl ether-47 while treatment with diallyl phthalate increased the level of fragmented-apoptotic cells (P=0.0283). Bisphenol A was the only agent that resulted only in induction of micronuclei but not any other kind of nuclear injuries.
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