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  • Modifying dipalmitoylphosphatidylcholine monolayers by n-hexadecanol and dipalmitoylglycerol.

Modifying dipalmitoylphosphatidylcholine monolayers by n-hexadecanol and dipalmitoylglycerol.

Chemistry and physics of lipids (2006-12-26)
Kerstin Wagner, Gerald Brezesinski
ABSTRACT

The monolayer structure of pure dipalmitoylphosphatidylcholine (DPPC) and equimolar mixtures of DPPC/n-hexadecanol (C(16)OH) and DPPC/dipalmitoylglycerol (DPG) are studied by the film balance technique and grazing incidence X-ray diffraction measurements. At 20 degrees C, the binary systems exhibit complete miscibility. In contrast to pure DPPC monolayers, a condensing effect is observed in the presence of both non-phospholipid additives; but the phase transition behavior differs. The tilt angle of the hydrocarbon chains in the DPPC/C(16)OH mixture is significantly smaller than in pure DPPC monolayers. The tilt of the chains is even further reduced in the mixed monolayer of DPPC/DPG. A comparison of the three systems reveals distinct structural features such as phase state, chain tilt, and molecular area over a wide range of surface pressures. Therefore, these monolayers provide a highly suitable model to investigate the influence of structural parameters on biological processes occurring at the membrane surface, e.g. enzymatic reactions and adsorption events.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1-Hexadecanol, ReagentPlus®, 99%
Sigma-Aldrich
Dipalmitin, ≥99.0%
Sigma-Aldrich
1-Hexadecanol, 95%
Sigma-Aldrich
1-Hexadecanol, ≥99%
Supelco
Cetyl Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Cetyl alcohol, analytical standard
Cetyl alcohol, European Pharmacopoeia (EP) Reference Standard