• Modifying dipalmitoylphosphatidylcholine monolayers by n-hexadecanol and dipalmitoylglycerol.

Modifying dipalmitoylphosphatidylcholine monolayers by n-hexadecanol and dipalmitoylglycerol.

Chemistry and physics of lipids (2006-12-26)
Kerstin Wagner, Gerald Brezesinski

The monolayer structure of pure dipalmitoylphosphatidylcholine (DPPC) and equimolar mixtures of DPPC/n-hexadecanol (C(16)OH) and DPPC/dipalmitoylglycerol (DPG) are studied by the film balance technique and grazing incidence X-ray diffraction measurements. At 20 degrees C, the binary systems exhibit complete miscibility. In contrast to pure DPPC monolayers, a condensing effect is observed in the presence of both non-phospholipid additives; but the phase transition behavior differs. The tilt angle of the hydrocarbon chains in the DPPC/C(16)OH mixture is significantly smaller than in pure DPPC monolayers. The tilt of the chains is even further reduced in the mixed monolayer of DPPC/DPG. A comparison of the three systems reveals distinct structural features such as phase state, chain tilt, and molecular area over a wide range of surface pressures. Therefore, these monolayers provide a highly suitable model to investigate the influence of structural parameters on biological processes occurring at the membrane surface, e.g. enzymatic reactions and adsorption events.

Product Number
Product Description

1-Hexadecanol, ReagentPlus®, 99%
Dipalmitin, ≥99.0%
1-Hexadecanol, 95%
1-Hexadecanol, ≥99%
Cetyl Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material
Cetyl alcohol, analytical standard
Cetyl alcohol, European Pharmacopoeia (EP) Reference Standard