MilliporeSigma
  • Home
  • Search Results
  • Increased proteolysis of collagen in an in vitro tensile overload tendon model.

Increased proteolysis of collagen in an in vitro tensile overload tendon model.

Annals of biomedical engineering (2007-09-04)
Thomas L Willett, Rosalind S Labow, Nicholas C Avery, J Michael Lee
ABSTRACT

Presently, there is a lack of fundamental understanding regarding changes in collagen's molecular state due to mechanical damage. The bovine tail tendon (BTT; steers approximately 30 months) was characterized and used as an in vitro model for investigating the effect of tensile mechanical overload on collagen susceptibility to proteolysis by acetyltrypsin and alpha-chymotrypsin. Two strain rates with a 1000-fold difference (0.01 and 10 s(-1)) were used, since molecular mechanisms that determine mechanical behavior were presumed to be strain rate dependent. First, it was determined that the BTTs were normal but immature tendons. Water content and collagen content (approx. 60% of wet weight and 80% of dry weight, respectively) and mechanical properties were all within the expected range. The collagen crosslinking was dominated by the intermediate crosslink hydroxylysinonorleucine. Second, tensile overload damage significantly enhanced proteolysis by acetyltrypsin and, to a lesser degree, by alpha-chymotrypsin. Interestingly, proteolysis by acetyltrypsin was greatest for specimens ruptured at 0.01 s(-1) and seemed to occur throughout the specimen. Understanding damage is important for insight into injuries (as in sports and trauma) and for better understanding of collagen fiber stability, durability, and damage mechanisms, aiding in the development of durable tissue-based products for mechanically demanding surgical applications.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Trypsin from porcine pancreas, tablet, 1 mg tablet
Sigma-Aldrich
Trypsin solution from porcine pancreas, 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
Sigma-Aldrich
Trypsin solution from porcine pancreas, sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
Sigma-Aldrich
TrypZean® bovine, recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid (USP)
Sigma-Aldrich
Trypsin from porcine pancreas, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture, 1,000-2,000 BAEE units/mg solid
Sigma-Aldrich
Trypsin from porcine pancreas, Type IX-S, lyophilized powder, 13,000-20,000 BAEE units/mg protein
Sigma-Aldrich
Trypsin from porcine pancreas, lyophilized powder, Type II-S, 1,000-2,000 units/mg dry solid
Sigma-Aldrich
Trypsin from porcine pancreas, lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid
Sigma-Aldrich
Trypsin solution from porcine pancreas, sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in 0.9% sodium chloride
SAFC
Trypsin-EDTA Solution 10X
SAFC
Trypsin Powder, Porcine 1:250, Gamma irradiated by SER-TAIN Process, dry powder
SAFC
Trypsin Solution 10X
Sigma-Aldrich
Trypsin from human pancreas, salt-free, lyophilized powder, vial of ≥1,000 BAEE units
Sigma-Aldrich
Trypsin from bovine pancreas, essentially salt-free, lyophilized powder, ≥9,000 BAEE units/mg protein, BioReagent, suitable for cell culture
Sigma-Aldrich
Trypsin from bovine pancreas, suitable for protein sequencing, lyophilized powder
Sigma-Aldrich
Trypsin from bovine pancreas, TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
Sigma-Aldrich
Trypsin from bovine pancreas, ≥2,500 USP units/mg solid, meets USP testing specifications
Sigma-Aldrich
Trypsin from bovine pancreas, Type XI, lyophilized powder, ≥6,000 BAEE units/mg protein
Sigma-Aldrich
Trypsin from bovine pancreas, Type I, ~10,000 BAEE units/mg protein
Sigma-Aldrich
Trypsin from bovine pancreas, TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
Sigma-Aldrich
Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid