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  • The effects of chromium VI on the fitness and on the beta-tubulin genes during in vivo development of the nematode Steinernema feltiae.

The effects of chromium VI on the fitness and on the beta-tubulin genes during in vivo development of the nematode Steinernema feltiae.

The Science of the total environment (2008-07-22)
Stephen Boyle, Thomais Kakouli-Duarte
ABSTRACT

The entomopathogenic nematode (EPN), Steinernema feltiae, is a commonly occurring nematode in the soil in Ireland. Consequently, we have conducted investigations as to the utility of this species as a candidate organism for the detection of chromium in Irish soils. These experiments have demonstrated that S. feltiae can survive and reproduce in the presence of high concentrations of chromium VI. It was observed that concentrations as high as 1000 ppm have little effect on the ability of this organism to produce large numbers of progeny. Nematodes were not observed to reproduce above 1800 ppm. However, an increase in development times for the nematode in vivo was noted at concentrations of 400 ppm upwards. This paper also illustrates the effects upon the beta-tubulin genes within nematode populations exposed to chromium VI in vivo. DNA sequencing has shown that elevated levels of variations occur among the population treatments, although these variations do not appear to be dependent upon chromium concentration. These findings constitute this organism appropriate for further investigation for the development of sub-lethal end points and biomarkers for the detection and biomonitoring of chromium VI contamination in soil.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium dichromate dihydrate, ACS reagent, ≥99.5%