• Home
  • Search Results
  • Novel fluorescence detection of free radicals generated in photolysis of fenvalerate.

Novel fluorescence detection of free radicals generated in photolysis of fenvalerate.

Journal of agricultural and food chemistry (2008-11-06)
Yusuke Suzuki, Toshiyuki Katagi
ABSTRACT

Photoinduced decarboxylation via homolytic cleavage of the ester linkage generating two benzyl radicals being recoupled is known to be a major photolytic pathway of the insecticide fenvalerate in aqueous or organic solvents. A highly sensitive and selective fluorescence spectroscopic method was applied to detect these radicals generated under xenon lamp irradiation in organic solvents and aqueous acetonitrile solutions. The short-lived radicals were efficiently trapped by the nitroxide free radical having a primary amino group, and the resultant diamagnetic adducts were instantaneously derivatized with fluorescamine as a fluorescent probe. The highly fluorescent derivatives were successfully separated and detected by a reversed-phase high-performance liquid chromatography equipped with a fluorescence detector, and their structures were individually identified by liquid chromatography/mass spectrometry.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Fluorescamine, ≥98% (TLC), powder, used for detection of primary amines
Sigma-Aldrich
Fluram, BioReagent, suitable for fluorescence, ≥99.0% (UV)

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.